Journal: iScience

Article Title: Patient-derived tumor organoids mimic treatment-induced DNA damage response in glioblastoma

doi: 10.1016/j.isci.2024.110604

Figure Lengend Snippet: Overview of the procedures used to generate GBOs from resected tumor tissue, cell-type-specific immunostaining of the GBOs and parental tumors and cytokine secretion from GBOs (A) Schematic presentation of main steps for generating GBOs from resected tumor tissue, created with bioRender.com . (B) Growth rate as the quantification of relative area of viable GBOs for five weeks. Data represent mean values ±S.E.M. ( n = 3 GBOs samples per patient (P)). (C) Immunofluorescence analyses of the GBO TME. GBOs express markers of GSCs (SOX2, CD9, and CD44) and TME cells, such as markers of cells of vasculature (CD31 and CD105), as well as macrophages and microglia (CD68 and Iba1). Representative images from a tissue sample from one patient are shown. Scale bar: 100 μm. (D) Selected cytokines and growth factors are secreted by GBOs in comparison to cultured GB cells from the same patient.

Article Snippet: Rabbit polyclonal to CD105 (ENG) , Atlas antibodies , Cat#HPA067440; RRID: AB_2685840.

Techniques: Immunostaining, Immunofluorescence, Comparison, Cell Culture

Journal: iScience

Article Title: Patient-derived tumor organoids mimic treatment-induced DNA damage response in glioblastoma

doi: 10.1016/j.isci.2024.110604

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal to CD105 (ENG) , Atlas antibodies , Cat#HPA067440; RRID: AB_2685840.

Techniques: Recombinant, Membrane, Blocking Assay, Red Blood Cell Lysis, Viability Assay, Reverse Transcription, Derivative Assay, Gene Expression, Software, Luminex